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Anatomical Sciences Journal، جلد ۶، شماره ۲۳، صفحات ۰-۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
Ulcert Repair by Spray of Epithelial Stem Cells |
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| چکیده انگلیسی مقاله |
Purpose: Separation, prolifration of stem cells and repairing of injured parts using these cells. Materials and Methods: This was a Lab-Experimental study. We used 6 male albino rabbits. At first, under general anesthesia 5x5 cm2 full thickness skin from one of the rabbits separated, washed using 70% alcohol and inserted in cold HBSS. Then it was cut into 4-6 mm pieces, washed again and incubated in Tripsin- EDTA 0.1% for 30 minutes at 37C. The cell suspention was then centrifuged and resuspended in DMEM culture. After 10 dayes the cells separated from the bed using Tripsin method. In experimental group the wounds were sprayed with 2 ml cultured keratinocytes cells and bandaged with vazeline. In control group just the skin was removed and the wounds healed withought cell spray. At the end of 4th week all rats were sacrificed, the repaired regions separated and studied with H&E and tricrom mason staining. Results: In cell studies, the colonies of stem cells were visible using special staining. In Histological studies the epiderm of repaired wounds with cells were normal but the keratoid layers were thiner than normal skin. No sweat gland was observed. Other findings were: shorter finger nodes, regular collagen fiber in dermal layer and wider vesseles. Conclusion: This method of cell culture would repaire a wide region and obtain a normal skin in a little time. |
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| کلیدواژههای انگلیسی مقاله |
Autologue, Skin, Rabbit, Cell Culture, Stem Cells |
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| نویسندگان مقاله |
| Manouchehr Safari
| Laya Ghahari
| Amir Khoushvaghti
| Ebrahim Nasiri
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| نشانی اینترنتی |
http://anatomyjournal.ir/browse.php?a_code=A-10-304-164&slc_lang=fa&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
Morphometry |
| نوع مقاله منتشر شده |
Original |
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