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Anatomical Sciences Journal، جلد ۵، شماره ۱۸، صفحات ۳۷-۴۷
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
The Effects of Lithium Chloride on Survival and Function of in Vitro Cultured Human Granulosa Cells |
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| چکیده انگلیسی مقاله |
Purpose: To investigate the effect of lithium chloride (LiCl) on survival and functional activity of in vitro cultured human granulosa cells (GCs). Materials and Methods: GCs were isolated from follicular fluid of women undergoing IVF/ICSI. After RBCs removal, GCs were plated in a medium containing varying concentrations of LiCl including 1, 2 and 5mM for two weeks period. A culture without LiCl was taken as control group. To induce apoptosis, the medium of all cultures, on day 14 were replaced by serum free media and the culture was extended for further 24 hour. At the end of cultivation period, apoptosis was assessed by TUNEL and flowcytometry analysis. Furthermore to assess secretional function of the cells in various groups, in some cultures, FSH and androstondion hormones were added and after 48 hours, subsequent secretion of 17-b estradiol was determined by RIA analysis. Results: The result showed that in GCs treated with 2 and 5 mM LiCl, apoptosis was statistically decreased more than 50% compared to control group (P>0.05) and estradiol secretion increased more than 40% in the GCs treated with 1,2,5 mM LiCl compared to control group. Conclusion: These results suggest that lithium increase functional activity of GCs and can protect GCs against apoptosis. |
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| کلیدواژههای انگلیسی مقاله |
Granulosa cells, Lithium chloride, Apoptosis, 17-beta estradiol |
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| نویسندگان مقاله |
| Bahareh Beyki
| Poopak Eftekhari Yazdi
Embryology Department, Royan Institute, Tehran, Iran.
| Bahman Zeynali
| mojtaba Reza Zadeh Valoujerdi
| Leyli Karimian
| Reza Salman Yazdi
| Ehsan Taghi Abadi
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| نشانی اینترنتی |
http://anatomyjournal.ir/browse.php?a_code=A-10-304-158&slc_lang=fa&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
Morphometry |
| نوع مقاله منتشر شده |
Original |
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