| چکیده انگلیسی مقاله |
Introduction: Cholinergic-associated diseases currently constitute a significant cause of neurological and neurodegenerative disabilities. As the drugs are not efficient in improving the suffered tissues, stem cell treatment is considered an effective strategy for substituting the lost cells. Methods: In the current study, we set out to investigate the differentiation properties of human Adipose-Derived Mesenchymal Stem Cells (AD-MSCs) into cholinergic-like cells by two morphogens of Retinoic Acid (RA) and Sonic Hedgehog (Shh) using a three-step in vitro procedure. The results were evaluated using real-time PCR, flow cytometry, and immunocytochemistry for two weeks. Results: Our data showed that the cells could express cholinergic specific markers, including Islet-1, Acetylcholinesterase (AChE), SMI-32, and Nestin, at mRNA and protein levels. We could also quantitatively evaluate the expression of Islet-1, AChE, and Nestin at 14 days post-induction using flow cytometry. Conclusion: Human AD-MSCs are potent cells to differentiate into cholinergic-like cells in the presence of RA and Shh through a three-step protocol. Thus, they could be a suitable cell candidate for the regeneration of cholinergic-associated diseases. However, more functional and electrophysiological analyses are needed in this regard. |
| نویسندگان مقاله |
| Davood Sanooghi
Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
| Naser Amini
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
| Fereshteh Azedi
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
| Zohreh Bagher
ENT and Head & Neck Research Center, The Five Basic Sensory Institute, Hazrat Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.
| Asghar Parvishan
Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
| Abolfazl Lotfi
Damavand Agricultural College, Technical and Vocational University, Tehran, Iran.
| Nooshin Rashidi
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
| Erfan Lotfi
School of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
| Forough Azam Sayahpour
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Academic Center for Education, Culture and Research, Tehran, Iran.
| Faezeh Faghihi
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
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