|
|
Basic and Clinical Neuroscience، جلد ۱۳، شماره ۵، صفحات ۰-۰
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژههای فارسی مقاله |
|
|
| عنوان انگلیسی |
Zanthoxylum Alatum Attenuates Chronic Restraint Stress Adverse Behavioral Effects via Mitigation of Oxidative Stress and Modulating the Expression of Genes Involved in Endoplasmic Reticulum Stress in Mice |
|
| چکیده انگلیسی مقاله |
Introduction: Endoplasmic reticulum (ER) serves many important functions particularly in the synthesis, folding, modification and transport of proteins. Based on the traditional claim and our previous studies on Zanthoxylum alatum in LPS- induced depressive behavior and scopolamine induced impaired memory, the present study was undertaken to investigate the role of hydro alcoholic extract of Zanthoxylum alatum (ZAHA) seeds in counteracting endoplasmic reticular stress in mice. Methods: The mice were restrained for a period of 28 days in polystyrene tubes. Two doses of ZAHA (100 and 200 mg/kg, p.o.) and the standard drug Imipramine (10mg/kg, i.p.) were administered daily, 45 min prior to restraint from day 22 to 28. Forced swim test and antioxidant enzymes levels, viz. superoxide dismutase (SOD), reduced glutathione (GSH) and lipid peroxidation (LPO) were estimated in the hippocampus of mice. Expression of genes, viz. 78 kilodalton Glucose Regulated Protein (GRP 78), 94 kilodalton Glucose Regulated Protein (GRP 94), C/EBP homologous protein (CHOP) by Real Time PCR were carried out to explore the molecular mechanism. Results: ZAHA (100 and 200 mg/kg, p.o. and Imipramine (i.p.) reverted stress by significantly reducing the immobility time in force swimming test, receding oxidative stress and lipid peroxidation. The antioxidant enzyme (SOD and GSH) levels were elevated than the restraint stress group. Down regulation of genes (GRP 78, GRP 94 and CHOP) in comparison to the chronic restraint stress group indicated stress modulating property of the seeds in ER stress. Hesperidin, Magnoflorine, Melicopine, Sesamin, isolated from the active extract, were hypothesized to exert the activity. Conclusion: It can be concluded that Zanthoxylum alatum reverted chronic restraint stress through anti-oxidant property and down regulation of few genes involved in endoplasmic reticulum stress. |
|
| کلیدواژههای انگلیسی مقاله |
Chronic restraint stress, Depression, Lipid peroxidation, Oxidative stress, Zanthoxylum alatum |
|
| نویسندگان مقاله |
| Chandana Choudhury Barua
Department of Pharmacology and Toxicology, College of Veterinary Science, Assam Agricultural University, Guwahati, Assam-781022, India.
| Lipika Buragohain
Department of Pharmacology and Toxicology, College of Veterinary Science, Assam Agricultural University, Guwahati, Assam-781022, India.
| Farida Rahman
Department of Pharmacology and Toxicology, College of Veterinary Science, Assam Agricultural University, Guwahati, Assam-781022, India.
| Ramakrishna Elancheran
Drug Discovery Lab, Life Science Division, Institute of Advanced Study in Science and Technology (IASST), West Boragaon, Garchuk, Guwahati-781035, Assam.
| Hooriyah Rizavi
Department of Psychiatry, Molecular Biology Research Building (MBRB), University of Illinois, IL, Chicago.
|
|
| نشانی اینترنتی |
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1477-1&slc_lang=en&sid=1 |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Behavioral Neuroscience |
| نوع مقاله منتشر شده |
Original |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|