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، جلد ۱۰، شماره ۱، صفحات ۰-۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Optimization of Culture Condition for the Production of Menaquinone-7 by Bacillus subtilis natto |
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| چکیده انگلیسی مقاله |
Background: Vitamin K2 refers to a series of naphthoquinone derivatives, which have a variety of physiological and pharmacological functions for the human body. The most important type of vitamin K2 is menaquinone-7 (MK-7), an expensive raw material with no local manufacturers in Iran. Objectives: Since there was no report on the yield of MK-7 produced by the currently available Bacillus subtilis natto species in Iran, this study aims to optimize the culture condition for the production of MK-7 using this Bacillus species. Methods: The base medium (BM) for MK-7 production contained glycerol (6.3%), soybean peptone (3%), and yeast extract (0.51%). The selected factors for optimizing the MK-7 production included the incubation temperature (30, 37, and 40°C) and incubation time (72, 96, and 120hr) with/without the addition of K2HPO4 to the fermentation medium. Three sets of experiments with six modes in each set were designed based on these parameters. MK-7 content was analyzed by the HPLC method. Results: Based on the designed experiments in this study, the highest MK-7 production yield were 0.319 and 0.3158 mg/L. These MK-7 yields were seen with adding K2HPO4 to medium and after 120hr incubation at 30 and 37°C, respectively. Conclusions: The results showed that Bacillus subtilis natto (IBRC-M 11153) is suitable to be used as a basic platform for mutation and production of a high-producer species. Optimizing the culture conditions using the wild-type species is not beneficial in increasing the production ability of the bacterium. This study would serve as a critical experimental basis for microbial production of MK-7 in Iran. However, it is necessary to use different methods for enhancing the production yield of MK-7 to lower the cost of microbial production and make the industrial process economic. |
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| کلیدواژه‌های انگلیسی مقاله |
Vitamin K2, Bacillus subtilis natto, Menaquinone-7, fermentation |
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| نویسندگان مقاله |
| Mohammad Sharifzadeh
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
| Negar Mottaghi-Dastjerdi
Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Iran University of Medical Sciences, Tehran, Iran
| Mohammad Soltany-Rezaee-Rad
Department of Pharmaceutical Biotechnology and Pharmaceutical Biotechnology Research Center, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
| Marjan Shariatpanahi
Department of Pharmacology and Toxicology, School of Pharmacy, Iran University of Medical Sciences, Tehran, Iran
| Israa Khalid AL-Yasari
Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Iran University of Medical Sciences, Tehran, Iran
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| نشانی اینترنتی |
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1239-1&slc_lang=en&sid=1 |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
بیوتکنولوژی |
| نوع مقاله منتشر شده |
پژوهشی |
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