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، جلد ۸، شماره ۴، صفحات ۲۰۱-۲۰۸
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Supplementation of Freezing Media with Cyclic Adenosine Monophosphate Analog and Isobutylmethylxanthine on Sperm Quality |
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| چکیده انگلیسی مقاله |
Background: This study aimed to explore whether the addition of a cyclic adenosine monophosphate (cAMP) analog and isobutylmethylxanthine (IBMX) in freezing media improved sperm quality and what role cAMP has in this recovery. Materials and methods: ach semen sample was cryopreserved into four groups: fresh semen sample, as a control group, freezing medium + 2.5 mM cAMP analog and 0.2 mM IBMX, freezing medium + 12.5 mM cAMP analog and 0.2 mM IBMX, and freezing medium + 25 mM cAMP analog and 0.2 mM IBMX. Sperm parameters after post-thaw were analyzed according to WHO instruction (2010). Viability, acrosome reaction, and DNA damage levels of the samples were evaluated. Results: Our results indicated that the effective concentrations of 12.5 and 25 mM cAMP analog and 0.2 mM IBMX significantly improved the total motility, progressive motility, and viability of the frozen-thawed (P< 0.05). However, non-progressive motility and immotile were significantly reduced in the 12.5 and 25 mM cAMP analogs and 0.2 mM IBMX groups after thawing (P< 0.05). During freezing the spermatozoa, the high concentration of the cAMP analog increased acrosome reaction after thawing in the 25 mM and 0.2 mM IBMX treated samples (P< 0.05). DNA fragmentation in 25 mM cAMP analog and 0.2 mM (IBMX) supplementation was significantly lower compared to the other groups (P< 0.05). Conclusions: Our findings revealed that in vitro cAMP analog and IBMX supplementation in freezing media play an important role in preventing cryodamage by maintaining the sperm functional parameters. |
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| کلیدواژه‌های انگلیسی مقاله |
cAMP analog (IBMX), cryopreservation, Asthenozoospermic, sperm quality |
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| نویسندگان مقاله |
| Rahil Jannatifar
Department of Reproductive Biology, the Academic Center for Education, Culture and Research, Qom branch, Iran
| Hamid Piroozmanesh
Department of Reproductive Biology, the Academic Center for Education, Culture and Research, Qom branch, Iran
| Leila Naserpoor
Department of Reproductive Biology, the Academic Center for Education, Culture and Research, Qom branch, Iran
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| نشانی اینترنتی |
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1147-1&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
بیولوژی |
| نوع مقاله منتشر شده |
پژوهشی |
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