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، جلد ۲۲، شماره ۶، صفحات ۱۳۶-۱۶۹
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| عنوان فارسی |
بررسی نظام‌مند مطالعات بیوفیزیکی اثر آفت‌کش‌ها بر تغییرات ساختاری پروتئین HSA به روش تجربی و محاسباتی |
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| چکیده فارسی مقاله |
Background and Aim : Human serum albumin (HSA) is one of the richest proteins in the vascular system that regulates transport and many chemical and molecular compounds. The aim of this study was to evaluate the effect of three groups of pesticides; Insecticides, fungicides and herbicides are based on changing the molecular structure of HSA. Materials and Methods: This systematic review covers 35 biophysical scientific-research studies related to the effect of pesticides on the structure of human serum albumin. These articles were selected through databases such as PabMed, Scopus, WebScience, Science Direct, and the Google Scholar search engine. The estimated time period was 1980 to 2019. Ethical considerations: In this study, all the principles of ethics in research have been observed. findings:Due to the close relationship between the biological activities of the HSA protein and its secondary structure, most articles have analyzed the secondary structure of the protein by various biophysical methods such as Fourier transform-infrared conversion, cyclic perspective and computational analysis. In general, it can be said that the pesticide-HSA interaction reduces the alpha-helix structure and increases other structures such as beta-sheet, beta-train and random coil. Most reports confirm that pesticides interact with HSA through hydrophobic, electrostatic, and hydrogen bond interactions. These interactions take place in subdomain IIA (Site One). The binding constant of pesticides to HSA protein was obtained in the range of 1-M103 to 106. Conclusion:Pesticide-protein interactions cause changes around the only major tryptophan of the ASH protein (Trp-214), alter IIA subdomain deformation, loss of normal structure, and decrease in free HSA concentrations, resulting in impaired transport of essential compounds such as drugs and vascular hormones. Creates. |
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| کلیدواژه‌های فارسی مقاله |
آلبومین سرم انسانی(HSA)، آفت‌کش‌ها، تعامل الکترواستاتیک، تعامل هیدروفوبیک، تبدیل فوریه ـ مادون قرمز، دورنگ‌نمایی دورانی |
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| عنوان انگلیسی |
A Systematic Review of The Biophysical Aspect of The Effect of Pesticides on The Structural Changes in HSA Protein: The Analysis of Experimental and Computational Studies |
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| چکیده انگلیسی مقاله |
Background and Aim: Human Serum Albumin (HSA) is one of the most abundant proteins in the blood vascular system which regulates the transportation of many chemical compounds and molecules. The purpose of this study is to review the studies about the effects of three groups of pesticides (Insecticides, herbicides and fungicides) on the molecular structure of HSA protein. Methods & Materials: This systematic review covers 35 studies of biophysical studies of the effect of pesticides on HSA protein. These papers were searched in PubMed, Science Direct, Web of Science databases and using Google Scholar search engine among those published from 1980 to 2019. Ethical Considerations: In this study, all ethical principles were considered. Results: Given the close relationship between biological activities of HSA and its secondary structure, the most of the reviewed articles analyzed the secondary structures of the HSA using various biophysical methods such as Fourier Transform Infrared (FTIR), Circular Dichroism (CD) and computational analysis. In general, HSA-pesticides interactions can cause a reduction in α-helix structure and an increase in other secondary structures including β-sheet, β-anti, and random coils. In the most reports, it has been proven that the pesticides interact with HSA through hydrophobic and electrostatic interactions and hydrogen bonding. These interactions take place in the IIA subdomain (Site 1) of HSA. The binding constants of these interactions were in the range of 10 3 to 10 6 M-1. Conclusion : The changes around the single important tryptophan residue of HSA (Trp-214) induce conformational deformity in the IIA subdomain of this protein which causes the loss of its native structure and leads to a decrease in free HSA concentrations which subsequently interrupts the transport of the essential compounds like drugs and hormones in the blood vascular system. |
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| کلیدواژه‌های انگلیسی مقاله |
Circular dichroism spectroscopy, Electrostatic interaction, Fourier transform infrared, Human serum albumin, Hydrophobic interaction. |
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| نویسندگان مقاله |
شهرزاد هادی چگنی | Shahrzad Hadi Chegni1
Department of Biophysics, Islamic Azad University, Science and Research Branch, Tehran, Iran.
گروه بیوفیزیک، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران، ایران.
محمد تقی زاده | Mohammad Taghizadeh
Department of Biotechnology, Faculty of Advanced Science and Technology, Faculty of Medical Sciences, Islamic Azad University, Tehran, Iran.
گروه بیوتکنولوژی، دانشکده علوم و فناوری پیشرفته، دانشکده علوم پزشکی، دانشگاه آزاد اسلامی، تهران، ایران
بهرام گلیایی | Bahram Goliaei
Department of Biophysics, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Iran
گروه بیوفیزیک، موسسه بیوشیمی و بیوفیزیک (IBB)، دانشگاه تهران، تهران.
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| نشانی اینترنتی |
http://jams.arakmu.ac.ir/browse.php?a_code=A-10-3519-2&slc_lang=fa&sid=1 |
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