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Hormozgan Medical Journal، جلد ۱۸، شماره ۲، صفحات ۰-۰
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| عنوان فارسی |
Isolation and identification of L-asparaginase from actinomycetes of Persian Gulf |
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| چکیده فارسی مقاله |
Introduction: L-asparaginase is an anti-neoblastic agent used in the chemotherapy of lymphoblastic leukemia. Although this enzyme is widely distributed among microorganisms, plants and animals, microorganisms have proved to be a better alternative, thus facilitating its large-scale production. Actinomycetes are filamentous, Gram-positive bacteria widely distributed in the marine environment. The aim of this study was isolation and molecular identification of L-asparaginase producing actinomycetes isolated from Persian Gulf. Methods: In this experimental study, 60 samples were collected from Persian Gulf in Hormozgan, Iran. All colonieses were screened for L-asparaginase activity with modified M9 medium. Lasparaginase activity was measured by colorimertic method. The strain with high L-asparaginase activity were selected and identified by nucleotide sequencing of 16S rRNA gene. Results: Thirty-two colonies of actinomycetes were obtained from sediment and seawater samples, among which 23 isolates were L-asparaginase producers. The strain PG08 with 37×10-2 IU activity showed the highest activity. Based on the analysis 16S rRNA gene sequences of this strain was showed maximum similarity to Streptomyces spp.OS1-33. Conclusion: Actinomycetes isolated from Persian Gulf may be potential source of enzyme Lasparaginase, Streptomyces spp. PG08 with high productivity L-asparaginase can be used for chemotherapy of acute lymphoblastic leukemia. |
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| کلیدواژه‌های فارسی مقاله |
Asparaginase،Actinomycetes،16S rRNA،Persian Gulf، |
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| عنوان انگلیسی |
Isolation and identification of L-asparaginase from actinomycetes of Persian Gulf |
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| چکیده انگلیسی مقاله |
Introduction: L-asparaginase is an anti-neoblastic agent used in the chemotherapy of lymphoblastic leukemia. Although this enzyme is widely distributed among microorganisms, plants and animals, microorganisms have proved to be a better alternative, thus facilitating its large-scale production. Actinomycetes are filamentous, Gram-positive bacteria widely distributed in the marine environment. The aim of this study was isolation and molecular identification of L-asparaginase producing actinomycetes isolated from Persian Gulf. Methods: In this experimental study, 60 samples were collected from Persian Gulf in Hormozgan, Iran. All colonieses were screened for L-asparaginase activity with modified M9 medium. Lasparaginase activity was measured by colorimertic method. The strain with high L-asparaginase activity were selected and identified by nucleotide sequencing of 16S rRNA gene. Results: Thirty-two colonies of actinomycetes were obtained from sediment and seawater samples, among which 23 isolates were L-asparaginase producers. The strain PG08 with 37×10-2 IU activity showed the highest activity. Based on the analysis 16S rRNA gene sequences of this strain was showed maximum similarity to Streptomyces spp.OS1-33. Conclusion: Actinomycetes isolated from Persian Gulf may be potential source of enzyme Lasparaginase, Streptomyces spp. PG08 with high productivity L-asparaginase can be used for chemotherapy of acute lymphoblastic leukemia. |
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| کلیدواژه‌های انگلیسی مقاله |
Asparaginase,Actinomycetes,16S rRNA,Persian Gulf |
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| نویسندگان مقاله |
| F. Izadpanah qeshmi
| S. Javadpour
| K. MalekZadeh
| S. Tamadoni Jahromi
| M. Rahimzadeh
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| نشانی اینترنتی |
http://hmj.hums.ac.ir/browse.php?a_code=A-10-2-1701&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
عمومی |
| نوع مقاله منتشر شده |
پژوهشی |
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