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تحقیقات دامپزشکی، جلد ۶۳، شماره ۲، صفحات ۱۴۱-۱۴۶
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| عنوان فارسی |
تشخیص آنتی ژن های توموری در عقده لمفاوی گاو مبتلا به لنفوسارکوم ویروسی با روش رسوب ایمنی |
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| چکیده فارسی مقاله |
In this study One lymph node from cow with viral lymphosarcoma and one lymph node from healthy cow were analyzed by three different methods for protein extraction, include simple homogenizing in PBS, sonication and phenol extraction. Tumor antigen detection relies on immunoprecipitation followed by SDS-PAGE. In this experiment Streptococcus pyogenes was used for purification of immune complexes. Eletrophoretic patterns were obtained using reduced and non reduced buffers. In PBS homogenization 3 distinct antigens (39, 32 and 30 kDa) were observed. When sonication or phenol extraction were used, 5 (72, 48, 42, 32 and 30 kDa) and 6 (104, 77, 54, 32, 30 and 26, 5 kDa) distinct antigens were observed, respectively. This study showed that the ability of antigen detection mainly depends on protein extraction procedure and antigen-antibody equilibrium achieved by quantification of precipitins. Immunoprecipitation could be used successfully for study of tumor antigens in bovine leukosis. |
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| کلیدواژههای فارسی مقاله |
آنتی ژن های توموری، رسوب ایمنی، لکوزگاوی، لمفوسارکوم، |
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| عنوان انگلیسی |
DETECTION OF TUMOR ANTIGENS IN BOVINE VIRAL LYMPHOSARCOMA BY IMMUNOPRECIPITATION METHOD |
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| چکیده انگلیسی مقاله |
In this study One lymph node from cow with viral lymphosarcoma and one lymph node from healthy cow were analyzed by three different methods for protein extraction, include simple homogenizing in PBS, sonication and phenol extraction. Tumor antigen detection relies on immunoprecipitation followed by SDS-PAGE. In this experiment Streptococcus pyogenes was used for purification of immune complexes. Eletrophoretic patterns were obtained using reduced and non reduced buffers. In PBS homogenization 3 distinct antigens (39, 32 and 30 kDa) were observed. When sonication or phenol extraction were used, 5 (72, 48, 42, 32 and 30 kDa) and 6 (104, 77, 54, 32, 30 and 26, 5 kDa) distinct antigens were observed, respectively. This study showed that the ability of antigen detection mainly depends on protein extraction procedure and antigen-antibody equilibrium achieved by quantification of precipitins. Immunoprecipitation could be used successfully for study of tumor antigens in bovine leukosis. |
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| کلیدواژههای انگلیسی مقاله |
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| نویسندگان مقاله |
غلامرضا نیکبخت بروجنی | nikbakht brujeni
گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
سید مهدی امام | seyed mehdi
گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
ندا برجسته |
گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
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| نشانی اینترنتی |
http://jvr.ut.ac.ir/article_23940_7164b59215bbed7c527dafc1f1e4015d.pdf |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/699/article-699-311527.pdf |
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| زبان مقاله منتشر شده |
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