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Basic and Clinical Neuroscience، جلد ۱۵، شماره ۶، صفحات ۰-۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Investigating the Possible Pretreatment Effect of Photo Biomodulation Through Alterations of MicroRNAs (21 and 124a) Expression in an Animal Model of Ischemic Stroke |
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| چکیده انگلیسی مقاله |
Introduction: The developing and promising optogenetic stimulation method can aid functional recovery by carefully regulating neuronal activity in brain circuits damaged by a stroke lesion. This investigation assesses the potential pretreatment effects of optogenetic stimulation on an ischemic stroke animal model.
Methods: Lentiviruses containing pLenti-CaMKIIa-hChR2 (H134R)-mCherry-WPRE were administered to adult male Wistar rats. It was injected into the right striatum for this purpose. Twenty-six days after the virus injection, the animals were exposed to blue laser light for six days in a row for 30 minutes at a time. Twenty-four hours after the final light stimulation, the transient middle cerebral artery occlusion (tMCAO) was done. One day after reperfusion, the neurological processes and the size of the brain infarcts in ischemic rats were evaluated. The transcript levels of microRNAs 21 and 124a—epigenetic indicators for neuroprotection and neurogenesis—were also assessed in the striatum and hippocampus.
Results: Our findings suggested that pretreatment with glutamatergic striatum optogenetic stimulation could reduce neurological impairments in rats and boost neuronal survival in both striatum and hippocampal regions. Also, the expression of microRNA-21 (miR-21) in the striatum was significantly increased in rats that had been optogenetically stimulated. Additionally, miR-124a expression was elevated in both regions in rats given tMCAO, and pretreatment with optical stimulations may considerably lower its expression in the hippocampus.
Conclusion: According to our findings, optogenetic stimulation pretreatment of the striatum positively affects stroke recovery. The effect is partially mediated by altering miRNAs involved in neurogenesis and subsequently activating its downstream signaling cascade. |
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| کلیدواژه‌های انگلیسی مقاله |
Ischemic stroke, Optogenetic stimulation, Ischemic tolerance, MicroRNA, Neuroprotection, Neurogenesis |
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| نویسندگان مقاله |
| Sanaz Mohammadi
Faculty of Biological Science and Technology, Shahid Beheshti University, Tehran, Iran.
| Salma Ahmadlou
Vaccination department, pasture institute of Iran, Tehran, Iran.
| Leila Dargahi
Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
| Mohammad Ismail Zibaii
Laser and Plasma Research Institute, Shahid Beheshti University, Tehran, Iran.
| Pouria Ghasemi
Laser and Plasma Research Institute, Shahid Beheshti University, Tehran, Iran.
| Afsaneh Asgari Taei
Neurobiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
| Andisheh Balouchi
Faculty of Biological Science and Technology, Shahid Beheshti University, Tehran, Iran.
| Mohammad Reza Bigdeli
Faculty of Biological Science and Technology, Shahid Beheshti University, Tehran, Iran.
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| نشانی اینترنتی |
http://bcn.iums.ac.ir/browse.php?a_code=A-10-4901-1&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Cellular and molecular Neuroscience |
| نوع مقاله منتشر شده |
Original |
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