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Immunoregulation، جلد ۱، شماره ۱، صفحات ۱-۸
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
Comparison of TGF-β and Nitric oxide production by adipose-derived mesenchymal stem cells between healthy pregnant and preeclamptic women |
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| چکیده انگلیسی مقاله |
Background: Preeclampsia is one of the most common complications of pregnancy that occurs after the 20th weeks of pregnancy.The pathophysiology of this disease is not exactly known. Transforming Growth Factor-beta (TGF-β) and Nitric Oxide (NO) are thekey regulatory factors secreted by Mesenchymal stem Cells (MSCs).Objectives: The aim of the present study was to evaluate the TGF-β and NO secretion by adipose-derived MSCs in normal andpreeclamptic pregnant women.Materials and methods: The adipose tissues were collected from 10 preeclamptic patients and 10 age-matched normotensivecontrols at the time of cesarean section delivery. After isolation and expansion of MSCs, their capability of differentiation andimmunophenotyping characteristics were assessed. Next, the release of TGF-ß was evaluated making use of ELISA sandwichmethod and Griess method was used to measure the level of NO.Results: Adipose derived MSCs in both groups were differentiated into osteocytes and adipocytes. The expression of CD90, CD73,CD44, and CD105 markers and lack of expression of CD-14, CD34, CD45, and HLA-DR markers in cells isolated from adipose in bothgroups was observed using flow cytometric analysis. The levels of TGF-ß secretion in preeclamptic women were significantly higherthan those in control group, but the mean level of NO secreted by adipose derived MSCs did not significantly change in the twogroups.Conclusion: It can be concluded that significant increase in the secretion of TGF-β owing to MSCs in preeclamptic participantsshows the importance of these cells in controlling immunological balance in these patients. Therefore, MSCs-based therapy seemsto regulate TH1/Th2 balance in preeclampsia. |
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| کلیدواژههای انگلیسی مقاله |
TGF-ß,NO,Mesenchymal stem cells,Adipose tissue,Preeclampsia |
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| نویسندگان مقاله |
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| نشانی اینترنتی |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
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