| چکیده انگلیسی مقاله |
Abstract Introduction: Non-fermentative Gram negative bacilli including some bactries are widely distributed in our environment. Despite of their low pathogen city potential, they are often the causative agents of infections in susceptible and immunocompromised patients. Objective: The study goals included: 1) determine of the prevalence of non-fermentative Gram negative bacilli among the total clinical specimens, 2) determine of the Genus and species of the bacterial isolation, and 3) determine of the relationship between different species isolation rate with the specific clinical specimen. Materials and Methods: During 11 months, clinical specimens were obtained from hospitalized patients in different hospitals in Tehran. Non-fermentative Gram negative bacilli were cultured from the specimens and their genus-species identity were determined microbiologically. The differentiation tests which have been used included: growth on Mac Conkey, TSI growth characteristics, oxidative fermentation of glucose and lactose, oxidize and motility tests, DNase, Nitrate reduction, Bile-esculin, and Indole reactions. Results: Of 6952 clinical specimens that were studied, 100(1.4%) were isolated of non-fermentative Gram negative bacilli. Fifty two of them were Pseudomonas aeroginosa, and the rest were members of other species 19 Alcaligenes faecalis (39.5%), 16 Acinetobacter baumannii (35.4%), 10 Acinetobacter lwoffi (20.8%), 2 Stenotrophomonas maltophilia (4.1%), 1 Flavobacterium meningosepticum (2%). Conclusion: Comparing the result of this study with similar investigations which were performed in other parts of the world, showed that: despite of the most encountered non-fermentative Gram negative bacteria isolates include, in order of prevalence, Pseudomonas aeroginosa, Alcaligenes faecalis and Acinetobacter baumannii respectively, but the rate of their prevalence are strikingly different in different hospitals and in different areas. Due to the widespread existence of antibiotic resistance in these bacteria their isolation, exact identification, and antibiotic susceptibility patterns are of paramount importance. |