Basic and Clinical Neuroscience، جلد ۷، شماره ۱، صفحات ۶۳-۷۳
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Focal Injection of Ethidium Bromide as a Simple Model to Study Cognitive Deficit and Its Improvement
چکیده انگلیسی مقاله Introduction: Memory and cognitive impairments are some of devastating outcomes of Multiple Sclerosis (MS) plaques in hippocampus, the gray matter part of the brain. The present study aimed to evaluate the intrahippocampal injection of Ethidium Bromide (EB) as a simple and focal model to assess cognition and gray matter demyelination. Methods: Thirty Wistar rats were divided into three groups: control group, which received saline, as solvent of EB, into the hippocampus and two experimental groups, which received 3 &muL of EB into the hippocampus, and then, were evaluated 7 and 28 days after EB injection (n=10 in each group), using a 5-day protocol of Morris Water Maze (MWM) task as well as Transmission Electron Microscopy (TEM) assay. Results: Seven days after EB injection, the behavioral study revealed a significance increase in travelled distance for platform finding in the experimental group compared to the control group. In addition, the nucleus of oligodendrocyte showed the typical clumped chromatin, probably attributed to apoptosis, and the myelin sheaths of some axons were unwrapped and disintegrated. Twentyeight days after EB injection, the traveled distance and the time spent in target quadrant significantly decreased and increased, respectively in experimental groups compared to the control group. Also, TEM micrographs revealed a thin layer of remyelination around the axons in 28 days lesion group. Discussion: While intracerebral or intraventricular injection of EB is disseminated in different parts of the brain and can affect the other motor and sensory systems, this model is confined locally and facilitates behavioral study. Also, this project could show improvement of memory function subsequent to the physiological repair of the gray matter of the hippocampus.
کلیدواژه‌های انگلیسی مقاله Behavior, Remyelination, Ethidium bromide, Hippocampus, Rat
نویسندگان مقاله مهدی گودرزوند | mahdi goudarzvand
department of physiology and pharmacology, school of medicine, alborz university of medical sciences, eshteraki ave., baghestan blv., karaj, iran.


سمیرا چوپانی | samira choopani
pasteur institute, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

علیرضا شمس | alireza shams
alborz university of medical sciences, karaj, iran


محمد جوان | mohammad javan
tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

زهره خدایی | zohreh khodaii
medical school, alborz university of medical sciences, karaj, iran


فرهاد قمصری | farhad ghamsari
alborz university of medical sciences, karaj, iran


ناصر نقدی | naser naghdi
pasteur institute, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

عباس پیریایی | abbas piryaei
shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

عباس حق پرست | abbas haghparast
shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

نشانی اینترنتی http://bcn.iums.ac.ir/browse.php?a_code=A-10-146-1&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Behavioral Neuroscience
نوع مقاله منتشر شده Original
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